Isolation ofRickettsia felisin the Mosquito Cell Line C6/36

Abstract
We report the isolation and establishment ofRickettsia felisin the C6/36 cell line. Rickettsial growth was intense, always with 90 to 100% of cells being infected after few weeks. The rickettsial isolate was confirmed by testing infected cells by PCR and sequencing fragments of three majorRickettsiagenes (gltA,ompB, and the 17-kDa protein gene).