A simplified method for isolating bacteria-free crown gall tissue from Vinca rosea
- 1 July 1971
- journal article
- Published by Canadian Science Publishing in Canadian Journal of Botany
- Vol. 49 (7) , 1255-1257
- https://doi.org/10.1139/b71-176
Abstract
A simplified procedure for the isolation of bacteria-free crown gall tissues requiring only a temperature-controlled water bath is described. Agrobacterium tumefaciens recovered from stem sections during this procedure retain their tumor-inducing properties as well as their antibiotic resistance.Keywords
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