Polyclonal Antibody Secretion Induced in Human Mixed Lymphocyte Cultures

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Abstract
In mixed leukocyte culture (MLC), using human spleen cells or thoracic duct lymphocytes, antibody secretion was induced and measured as plaque-forming cells (PFC) in a hemolysis-in-gel assay with fluorescein isothiocyanate (FITC)-coupled sheep erythrocytes (SRBC) as targets. Peak antibody secretion was seen on day 5. Using protein-A-coupled SRBC as targets and developing antisera, antibody secretion in MLC was of Ig[immunoglobulin]M, IgG and IgA type. There was no correlation between the number of PFC against FITC-SRBC in MLC and DNA synthesis. Supernatants from MLC failed to induce antibody secretion.