Analog Inhibitors for the Pyrophosphate-Dependent Phosphofructokinase of Entamoeba histolytica and their Effect on Culture Growth

Abstract
Diphosphonate substrate-analogs of inorganic pyrophosphate (PPi) [1,1-diphosphonate, nonane, ethylene, hydroxymethane, 2-aminoethane and 1-hydroxyethane] were found to be competitive inhibitors for amoebal PPi-dependent phosphofructokinase. Analogs of the phosphoryl donor were employed in in vitro growth tests. All appeared to inhibit amoebal growth; one was amoebistatic at a concentration of 1 mM. Pyrophosphate analogs are, potentially, therapeutic agents for intestinal amoebiasis [in humans].

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