Minimal antigenic characterization of eight Rhizobium meliloti strains by indirect enzyme-linked immunosorbent assay (ELISA)

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Abstract
The somatic antigens of eight Rhizobium meliloti strains were characterized by indirect enzyme-linked immunosorbent assay (ELISA) carried out in microtiter plates. Washed cells were bound by evaporation of cell suspension in plate wells at 85 °C. The antigenic characterization was based on determinations of the selective removal of antibodies from antisera against the strains by extensive reciprocal adsorptions with heterologous cells. Four of the strains were found to possess distinctive antigens and a total of seven different combinations of antigens were found among the eight strains. One pair of the strains was not distinguishable by the assay used.