Some Physico-chemical Properties of the Photo-induced Semiquinone of D-Amino Acid Oxidase and Influence of Pyruvate and Phenylpyruvate upon Its Formation

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Abstract
1. The absorption spectrum of the photo-induced semiquinone of D-amino acid oxidase [EC 1.4.3.3] was calculated. The spectrum coincided with that of the dithionite reduced semiquinone. 2. The E′o of photo-induced semiquinone was estimated as approximately—-125 mV. The semiquinone reduced cytochrome c in an anaerobic condition. 3. D-Amino acid oxidase combined with pyruvate or phenylpyruvate did not form the photo-induced semiquinone by irradiation under anaerobic conditions, but turned to colorless form. This colorless form was not autoxidizablc and contained undecomposed flavin.