Recognition of the Nucleoside in the First Position of the Anticodon of Isoleucine tRNA by Isoleucyl-tRNA Synthetase from Escherichia Coli
- 1 February 1992
- journal article
- research article
- Published by Taylor & Francis in Nucleosides and Nucleotides
- Vol. 11 (2-4) , 719-730
- https://doi.org/10.1080/07328319208021736
Abstract
Escherichia coli tRNAIle 1 prepared in vitro by T7 RNA polymerase transcription was found to be charged with isoleucine by E. coli isoleucyl-tRNA synthetase (IleRS). Replacement of G in the first position of the anticodon with U, C, or A resulted in complete loss of isoleucine-accepting activity. This indicates that IleRS strictly recognizes the structural feature of guanosine in this position of tRNAIle 1 which is common to that of lysidine in E. coli tRNAIle 2.Keywords
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