Electroblot analysis of the myelin proteolipid protein
- 1 January 1982
- journal article
- research article
- Published by Wiley in Journal of Neuroscience Research
- Vol. 7 (1) , 1-10
- https://doi.org/10.1002/jnr.490070102
Abstract
The myelin proteolipid has been studied by the electroblot method of Towbin et al [1979]. Samples were separated by SDS–polyacrylamide gel electrophoresis, transferred to nitrocellulose, and incubated with proteolipid antibody. The proteolipid band could be identified immunologically in CNS myelin and in whole brain homogenates. No proteolipid was detected in PNS myelin. Proteolipid from human, bovine, rat, and mouse myelin all cross‐react when analyzed by this method. No cross‐reactivity was indicated between proteolipid and myelin basic protein.Keywords
This publication has 18 references indexed in Scilit:
- Developmental regulation of myelin basic protein in dispersed culturesProceedings of the National Academy of Sciences, 1981
- PURIFICATION AND COMPARISON OF 2′,3′‐CYCLIC NUCLEOTIDE 3′‐PHOSPHOHYDROLASES FROM BOVINE BRAIN AND SPINAL CORDJournal of Neurochemistry, 1978
- Identification of prelarge and presmall basic proteins in mouse myelin and their structural relationship to large and small basic proteinsProceedings of the National Academy of Sciences, 1977
- Proteins of MyelinPublished by Springer Nature ,1977
- Gel electrophoresis studies of bovine brain white matter proteolipid and myelin proteinsBiochemistry, 1974
- PROTEOLIPIDS FROM MEMBRANE SYSTEMS*Annals of the New York Academy of Sciences, 1972
- Modification of the lowry procedure for the analysis of proteolipid proteinAnalytical Biochemistry, 1972
- Isolation of a highly purified myelin proteinBiochemistry, 1971
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970
- Maturation of human white matter myelin. Fractionation of the myelin membrane proteinsBiochemistry, 1968