PROTEINS IN THE COLLOIDAL GOLD REACTION

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Abstract
Exptl. data showing the individual effects of blood fractions on uniformly prepared colloidal Au sols at pH 7.4 are presented which confirm the coagulatory action of [gamma]-globulin and which show that one or more [beta]-globulins (and possibly [alpha]-globulins) are responsible for the protective action of normal body fluids as distinct from the serum albumin which is inert. The use of [gamma]-globulin in determining whether or not two batches of Au sol are comparable is suggested. Conversely, a standardized Au sol may be found useful in the analysis of blood fractions. In a discussion of the implications of these results, hypotheses are advanced to account for the usual types of colloidal Au reactions exhibited by body fluids in a milieu buffered at pH 7.4.