A maize pectin methylesterase-like gene, ZmC5, specifically expressed in pollen
- 1 January 1998
- journal article
- Published by Springer Nature in Plant Molecular Biology
- Vol. 37 (1) , 187-192
- https://doi.org/10.1023/a:1005954621558
Abstract
Pectin methylesterase (PME) is responsible for the demethylation of pectin prior to pectin's degradation by the combined activities of polygalacturonase and pectate lyase. We have differentially screened a maize pollen cDNA library to detect cDNA clones whose genes are specifically expressed in pollen. One group of clones resulting from this screen showed similarity (between 18% and 41% identity) with plant and fungal PMEs. The full-length clone from this group, ZmC5, identifies a small gene family (at least 2 members) when used as a probe on genomic Southern blots. Northern analysis reveals that the ZmC5 transcript is expressed specifically in late pollen development. This tissue-specific gene expression programme is further confirmed in transgenic tobacco plants harbouring ZmC5 promoter/GUS chimeric gene constructs.Keywords
This publication has 21 references indexed in Scilit:
- Four members of the maize β‐tubulin gene family are expressed in the male gametophyteThe Plant Journal, 1993
- Characterization and overexpression of the pem gene encoding pectin methylesterase of Erwinia chrysanthemi strain 3937Gene, 1993
- Transgene copy number can be positively or negatively associated with transgene expressionPlant Molecular Biology, 1993
- Disulfide bridges in tomato pectinesterase: Variations from pectinesterases of other species; conservation of possible active site segmentsProtein Science, 1992
- Regulation of the Osmotin Gene Promoter.Plant Cell, 1992
- A gene showing sequence similarity to pectin esterase is specifically expressed in developing pollen of Brassica napus. Sequences in its 5? flanking region are conserved in other pollen-specific promotersPlant Molecular Biology, 1991
- Molecular cloning and sequencing of a pectinesterase gene from Pseudomonas solanacearumMicrobiology, 1991
- Upstream sequences other than AAUAAA are required for efficient messenger RNA 3'-end formation in plants.Plant Cell, 1990
- [20] Computer prediction of RNA structurePublished by Elsevier ,1989
- Mode of action of pectic enzymes. III. Site of initial action of tomato pectinesterase on highly esterified pectinBiochemistry, 1970