Studies of DEAE‐dextran‐mediated gene transfer
- 1 February 1997
- journal article
- Published by Wiley in Biotechnology and Applied Biochemistry
- Vol. 25 (1) , 47-51
- https://doi.org/10.1111/j.1470-8744.1997.tb00413.x
Abstract
DEAE-dextran-mediated gene transfer was studied for the introduction of pSV2neo DNA into Fisher-rat 3T3 (FR3T3) cells. Zeta (z) potentials of the DEAE-dextranŐDNA complexes and FR3T3 cells were found to be dependent on the concentration of DEAE-dextran in the medium. The maximum transfection efficiency occurred at a DEAE-dextran/DNA ratio of 50:1 or thereabouts. The interaction between DNA and cells is determined by the adsorption process. The results obtained, along with the correlation between the kinetic adsorption behaviour of 3H-labelled DNA and the transfection efficiency, indicated that adsorption of DEAE-dextranŐDNA complexes to the negatively charged cell surfaces, due to electrostatic and dispersion attraction, plays the decisive role in determining the DNA transfection efficiencies.Keywords
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