Partial Cloning of Prohibitin cDNA from Canine, Feline, Bovine, Equine, and Rabbit Liver mRNA by RT-PCR.
- 1 January 1997
- journal article
- research article
- Published by Japanese Society of Veterinary Science in The Journal of Veterinary Medical Science
- Vol. 59 (3) , 201-203
- https://doi.org/10.1292/jvms.59.201
Abstract
Prohibitin is the protein which has an inhibitory function in cell growth, and its gene is suggested to be one of putative tumor suppressor genes. In this report, we described a partial cloning of prohibitin cDNAs from canine, feline, bovine, equine, and rabbit liver mRNAs by RT-PCR, and their homology analysis. The sequences of these RT-PCR products were compared with each other as well as those reported for human and rat. The homology in this region of prohibitin cDNA was approximately 90%, and the amino acid sequence of each RT-PCR product shared more than 95% identity. Therefore, it is concluded that all the RT-PCR products are a part of prohibitin cDNA of each animal.Keywords
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