Effect of Quick Freezing at Very Low Temperatures of Donor Tissue in Gorneal Transplants.

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Abstract
Fifteen corneal transplantations were performed on rabbits by Castroviejo''s method. The donor tissue was rabbit corneas frozen in either liquid iso-pentane chilled with liquid N, or liquid N alone, and rapidly thawed just prior to implantation. A control series consisting of 21 keratoplasties was made with living donor tissue. The same operating technique and post-operative treatment was used in both sets of experiments. The results showed 13 perfectly healed transparent grafts in the series where living corneal tissue was used as donor material, with 8 nebulous, incompletely healed, or opaque transplants. All of the grafts made from frozen corneas were healed perfectly after a slight delay, but became opaque and vascular. These results support the theory that living tissue is required for the production of permanently transparent grafts and that the corneal strotna of a clear graft retains its identity as donor tissue for at least a long period of time.

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