Mismatch repair detection (MRD): high-throughput scanning for DNA variations
Open Access
- 1 August 2001
- journal article
- research article
- Published by Oxford University Press (OUP) in Human Molecular Genetics
- Vol. 10 (16) , 1657-1664
- https://doi.org/10.1093/hmg/10.16.1657
Abstract
Although there are several methods for genotyping previously identified single nucleotide polymorphisms (SNPs), there is a paucity of approaches for high-throughput scanning for unknown variations. Mismatch repair detection (MRD) utilizes a bacterial mismatch repair system in vivo to detect sequence variants in human DNA samples. We describe modifications in MRD that allow a high degree of parallel processing, and use this modified version to accurately scan for variations in 35 different human DNA fragments simultaneously. MRD’s potential for high-throughput scanning can be used to identify new SNPs and to comprehensively compare sequences between patients and controls for identifying disease susceptibility alleles.Keywords
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