Preparative High-Performance Liquid Chromatographic Separation of Individual Amino Acids Derived from Fossil Bone Collagen

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Abstract
Extracting a series of amino acids from fossil “collagen” makes it possible to compare chemically well-characterized molecules from one bone sample, both chemically and by isotopic analysis. Thus, the integrity of collagen samples and possible contamination can be checked. We describe the routine high-performance liquid chromatographic extraction of five amino acids: glycine, hydroxyproline, glutamate, threonine and alanine. Humates are eliminated during the extraction, which can be monitored continuously.