Use of antioxidants in the analysis of vatamins A and E in mammalian plasma by high performance liquid chromatography
- 1 November 1983
- Vol. 18 (11) , 837-841
- https://doi.org/10.1007/bf02534644
Abstract
A simple, sensitive, quantitative method for the simultaneous assay of retinol, α‐tocopherol and γ‐tocopherol in rat, guinea pig, monkey and human plasma was developed by using high performance liquid chromatography. It was found that antioxidant was required to stabilize the fat‐soluble vitamins in the plasma of rats. The effect of several antioxidants on the recovery of fat‐soluble vitamins was evaluated. Results showed that 0.125% butylated hydroxytoluene (BHT) in ethanol and 0.025% BHT in heptane yielded recoveries >95% in 0.1 ml plasma.This publication has 11 references indexed in Scilit:
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