Determination of ofloxacin in blood by high-performance liquid chromatography.
- 1 January 1988
- journal article
- Published by Japanese Society of Pharmaceutical Health Care and Sciences in Japanese Journal of Hospital Pharmacy
- Vol. 14 (5) , 323-327
- https://doi.org/10.5649/jjphcs1975.14.323
Abstract
Ofloxacin (OFLX) is one of the oral antibacterial agents belongs to pyridone carbonates.We assayed OFLX by high-performance liquid chromatography (HPLC) using the deproteinization with acetonitrile for pretreatment of serum.Mobile phase consisted of 40mM H3PO4 and 10mM KH2PO4 in 90% acetonitrile-water (pH 3.5).We used DL-8357 as an internal standard (I. S.) Serum (20μl) was taken in the polyethylene tube, and 200μl of the acetonitrile containing I.S. was added into the tube, and the tube was centrifuged for 1 min mixing. After the centrifugation 25μl of the supernatant was injected into the HPLC.This method is a simple and required short time, and its producibility was good.Keywords
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