Human peroxiredoxin 5 is a peroxynitrite reductase
- 10 July 2004
- journal article
- Published by Wiley in FEBS Letters
- Vol. 571 (1-3) , 161-165
- https://doi.org/10.1016/j.febslet.2004.06.080
Abstract
Peroxiredoxins are an ubiquitous family of peroxidases widely distributed among prokaryotes and eukaryotes. Peroxiredoxin 5, which is the last discovered mammalian member, was previously shown to reduce peroxides with the use of reducing equivalents derived from thioredoxin. We report here that human peroxiredoxin 5 is also a peroxynitrite reductase. Analysis of peroxiredoxin 5 mutants, in which each of the cysteine residues was mutated, suggests that the nucleophilic attack on the O-O bond of peroxynitrite is performed by the N-terminal peroxidatic Cys(47). Moreover, with the use of pulse radiolysis, we show that human peroxiredoxin 5 reduces peroxynitrite with an unequalled high rate constant of (7+/-3)x10(7) M(-1)s(-1).Keywords
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