The role of the transforming a gene of SV40 in the mutagenic activity of the virus
- 1 October 1982
- journal article
- research article
- Published by Springer Nature in Molecular Genetics and Genomics
- Vol. 187 (3) , 473-476
- https://doi.org/10.1007/bf00332631
Abstract
The mutagenic activity of the tsA239 mutant of SV40 which synthetizes a defective T antigen at 40°C was investigated in Chinese hamster cells under permissive and nonpermissive temperature. At 33°C the virus increased the yield of 6-mercaptopurine-resistant colonies after 2 days expression time by a factor of 1.6–4 as compared with the control and raised the frequency of aberrant metaphases after the same time by a factor of 1.9–3.4. In the same experiments, with the same initially infected population of Chinese hamster cells, at 40°C tsA SV40 did not induce either gene mutations or chromosome aberrations at the same early stage after infection. Presumably the activity of the A gene of SV40 is necessary not only for the transforming but also for the mutagenic effect of the virus.This publication has 23 references indexed in Scilit:
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