Direct measurement of the hindquarter blood flow was obtained in an acute preparation in which the inferior vena cava was cannulated just distal to the renal veins. Following the intravenous administration of .02 mg. glucagon/kg., to dogs in a post-absorptive state, there was a marked decrease in hindquarter blood flow. A mean decrease to 52% of control values occurred between 10 and 20 minutes after glucagon. This effect was not observed in a dog fasted 5 days. Administration of glucose, in amounts roughly comparable to the amount of glucose released by the liver with this dose of glucagon, produced a transient increase in hindquarter flow. These findings, together with previously reported findings of increased hepatic blood flow, suggest a redistribution of cardiac output following glucagon.