GROWTH SUPPORT OF SMALL B16 MELANOMA IMPLANTS WITH NITROSOUREA-STERILIZED FRACTIONS OF SAME TUMOR

  • 1 January 1976
    • journal article
    • research article
    • Vol. 36  (6) , 2031-2034
Abstract
B16 melanoma cells sterilized in vitro with 1,3-bis(2-chloroethyl)-1-nitrosourea or in vivo with trans-1-(2-chloroethyl)-3-(4-methylcyclohexyl)-1-nitrosourea, were used to enhance the percentage of tumor takes with small s.c. implants of viable cells and reduce the latent period between tumor implantation and palpability. The admixture of trans-1-(2-chloroethyl)-3-(4-methylcyclohexyl)-1-nitrosourea-inactivated cells with viable cell implants reduced the number of cells required to produce tumors in 50% of the mice by approximately 3 log10 units and reduced the time of tumor appearance from implants of up to 106 cells. Similar results were obtained with 1,3-bis(2-chloroethyl)-1-nitrosourea-sterilized cells. The growth supporting effect obtained with the nitrosourea-inactivated cells appeared to be as pronounced as that previously reported, for this tumor system, with radiation-inactivated cells.

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