A Method for the Gas Chromatographic Determination of Vitamin D and Related Structures
- 1 September 1976
- journal article
- research article
- Published by Oxford University Press (OUP) in Journal of Chromatographic Science
- Vol. 14 (9) , 434-437
- https://doi.org/10.1093/chromsci/14.9.434
Abstract
A procedure is described for the separation of Vitamin D and related compounds by gas-liquid chromatography using flame ionization detection. The method involves a two-step conversion: isomerization to the corresponding (all trans) isotachysterol(s) followed by methyl ether derivatization of the alcohol group(s). The procedure provides a means for identification as well as a possible basis for quantitation of Vitamin D and analogs.Keywords
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