Uptake and oxidation of malonaldehyde by cultured mammalian cells

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Abstract
Primary cultures of rat skin fibroblasts were used as a model system to investigate the cellular uptake and oxidation of malonaldehyde (MA). The cells were grown in a medium containing 10−5 M, 10−4 M or 10−3 M concentrations of [1,3-14C]MA. There was a limited, concentration-dependent uptake of MA by 24 hr (∼4% at all concentrations). The uptake of [1,2-14C]acetate by 24 hr was ∼24%; 83–89% of the14C in the MA taken up was oxidized to14CO2 by 24 hr and ∼5% was recovered in the major lipids. Despite its low uptake and rapid oxidation to CO2, pretreatment of the cells with 10−3 M MA for 24 hr produced a latent inhibition of [14C]glucose oxidation. Limited cellular uptake of MA may explain the tolerance of cells grown in culture to relatively high MA concentrations.