Quantitative assays for uracil-DNA glycosylase of high sensitivity
- 1 February 1988
- journal article
- research article
- Published by Canadian Science Publishing in Biochemistry and Cell Biology
- Vol. 66 (2) , 157-160
- https://doi.org/10.1139/o88-021
Abstract
We have developed a sensitive fluorometric assay using bisulfite deaminated (C → U), covalently-closed circular PM2 DNA as the substrate. We describe a reliable way to prepare this substrate without nicking the PM2 DNA. Methods, which depend on toluenization of the cells, are described for reproducibly and quantitatively assaying uracil-DNA glycosylase. The sensitivity is such that only 200 EL4 mouse thymoma cells or 30 000 Escherichia coli cells are needed for each point in a kinetic assay.This publication has 5 references indexed in Scilit:
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