CHARACTERIZATION OF ANTIGEN-PRESENTING ACTIVITY OF INTESTINAL MONONUCLEAR-CELLS ISOLATED FROM NORMAL AND INFLAMMATORY BOWEL-DISEASE COLON AND ILEUM

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  • 1 December 1988
    • journal article
    • research article
    • Vol. 65  (4) , 543-549
Abstract
Antigen-presenting activity in mononuclear cells, isolated form normal and inflamed human ileum and colon, has been characterized using allogeneic mixed lymphocyte reaction with resting T cell as responders. Greatest proliferation was induced by fibronectin-adherent (macrophage-enriched) cells, and least by fibronectin non-adherent (macrophage-depleted) cells and by mononuclear cells depleted of macrophages by panning with monoclonal antibody 3C10. When intestinal mononuclear cells and alogeneic T cells were incubated in large numbers, clusters were observed. These clusters contained cells with a dendritic morphology that were strongly HLA-D-positive and which also stained with macrophage-specific monoclonal antibodies 3C10, EMB11 adn Y1/82A. These cells were closely associated with proliferating T cells. Studies comparing mononuclear cells isolated from normal and inflamed colonic mucosa suggest that the latter may have enhanced antigen-presenting capacity.

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