In vitro activation of dacarbazine (DTIC) for a human tumor cloning system

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Abstract
Dacarbazine (DTIC) is an agent with clinical activity against human malignant melanoma. We have explored two methods for activating DTIC so it may be used in vitro in a human tumor cloning system. The activation of DTIC by white light was found to be a viable alternative to utilizing a microsome plus cofactor system for bioactivation. The microsome plus cofactor system itself actually caused some inhibition of tumor colony formation. Light activation of DTIC appears to be a reliable and simple method which allows testing of DTIC in an in vitro soft agar culture system.