A new method for assay of ferroxidase activity and its application to human and rabbit sera.

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Abstract
A simple, sensitive and precise method for serum ferroxidase assay was developed by determining Fe(III) produced enzymatically from Fe(II) added as a substrate in an acetate buffer (pH 6.0). The Fe(III) formed was determined by flow injection analysis (FIA) using thiocyanate as a detection reagent. The reproducibility of this method throughout all steps was satisfactory (coefficient of variation, C.V. = 3.4%), and the normal value of human serum ferroxidase activity was 0.30 .+-. 0.02 .mu.mol Fe(III)/min per ml (mean .+-. SD). This method was applied to study the behavior of human and rabbit serum ferroxidases on Sephadex G-150 column chromatography.