Isolation of a Second Rifamycin‐Binding from Escherichia coli by Affinity Chromatography
- 1 June 1981
- journal article
- Published by Wiley in European Journal of Biochemistry
- Vol. 117 (1) , 87-91
- https://doi.org/10.1111/j.1432-1033.1981.tb06306.x
Abstract
When extracts of Escherichia coli are filtered through a Sepharose column containing covalently bound rifamycin a protein is bound which can be eluted either with a high concentration of urea or more specifically with low concentrations of rifamycins. Its Mr is 18000 ± 1000 in the presence of dodecylsulfate, in its absence 36000 ± 3000. The association constant of the protein for rifampicin is 2.4 ± 0.5 × 10−4 M with two binding sites per dimer as determined by equilibrium dialysis. Large amounts of this protein are released from the cells by an osmotic shock.Keywords
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