Induction of epstein‐barr virus by a new tumor promoter, teleocidin, compared to induction by TPA

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Abstract
The effect of teleocidin, a new, naturally occurring tumor promoter, on induction of Epstein‐Barr virus (EBV), was compared with that of a known tumor promoter, 12‐O‐tetradecanoyl‐phorbol‐13‐acetate (TPA). Early antigen (EA) and/or capsid antigen (VCA) of EBV was induced in the EBV genome‐carrying cell lines C‐6 and P3HR‐I cells by teleocidin, its effect being maximal at a concentration of 12.5 ng/ml. The production of infectious EBV from P3HR‐I cells was enhanced by teleocidin maximally at a concentration of 0.5 to 2.5 ng/ml. The outgrowth of EBV‐transformed cells from peripheral lymphocytes of seropositive healthy donors was also enhanced by teleocidin at a concentration of 0.02 to 0.5 ng/ml. TPA tested simultaneously in all experiments exhibited the same activities as teleocidin, and was effective at similar concentrations. Teleocidin enhanced both EA and VCA synthesis in P3HR‐I cells additively with n‐butyrate, but not with TPA. This suggests that teleocidin and TPA have a common mechanism of action, although their chemical structures are different.