Cell autonomous expression of IgD is not essential for the maturation of conventional B cells
- 1 December 1991
- journal article
- research article
- Published by Oxford University Press (OUP) in International Immunology
- Vol. 3 (12) , 1367-1371
- https://doi.org/10.1093/intimm/3.12.1367
Abstract
To analyse the function of IgD in vivo, we generated a ‘loss of function’ mouse model utilizing gene targeting technology. By homologous recombination in a (C57BL/6 × CBA)F1 mouse embryonic stem cell (ES) line one allele of the δ heavy chain gene was rendered non-functional. In chimeric mice obtained after injection of the targeted ES cells into blastocysts derived from severe combined immunodeficient mice we analysed ES cell derived B lymphocytes expressing the targeted or the wild-type allele by using allotype specific reagents. We show that B cells expressing the targeted allele appear in the periphery as lgM+D− cells at normal frequency. They express the CD23 marker and respond to a T cell dependent antigen. Thus, cell autonomous expression of IgD is neither essential for B cell maturation into an antigen responsive state nor for antigen dependent triggering of the cells into an immune response.Keywords
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