Sex ratio studies were made on bluegill (Lepomis macrochirus) in: 2 pond populations containing fish from the National Fish Hatchery, Marion, Alabama; a farm pond of the Agriculture Experiment Station, Auburn University; Lake Martin of the Alabama River System; and Lake Eufaula of the Chattahoochee River System. Each population contained a significantly greater number of females than males at probability 0.01. Of a total 4456 bluegill examined from the 4 populations, 1383 were males and 3073 were females - a 1: 2.2 male to female ratio.