Cell‐Free Synthesis of the Mitochondrial ADP/ATP Carrier Protein of Neurospora crassa

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Abstract
ADP/ATP carrier protein was synthesized in heterologous cell-free systems programmed with Neurospora poly(A)-containing RNA and homologous cell-free systems from Neurospora. The apparent MW of the product obtained in vitro was the same as that of the authentic mitochondrial protein. The primary translation product obtained in reticulocyte lysates starts with formylmethionine when formylated initiator methionyl-tRNA (fMet-tRNA/fMet) was present. The product synthesized in vitro was released from the ribosomes into the postribosomal supernatant. The ADP/ATP carrier is apparently synthesized as a polypeptide with the same MW as the mature monomeric protein and does not carry an additional sequence.