Restoration of Anti-Human Immunodeficiency Virus Type 1 (HIV-1) Responses in CD8+T Cells from Late-Stage Patients on Prolonged Antiretroviral Therapy by Stimulation In Vitro with HIV-1 Protein-Loaded Dendritic Cells
Open Access
- 1 May 2001
- journal article
- research article
- Published by American Society for Microbiology in Journal of Virology
- Vol. 75 (9) , 4413-4419
- https://doi.org/10.1128/jvi.75.9.4413-4419.2001
Abstract
We demonstrate that dendritic cells loaded in vitro with human immunodeficiency virus type 1 (HIV-1) protein-liposome complexes activate HLA class I-restricted anti-HIV-1 cytotoxic T-lymphocyte and gamma interferon (IFN-γ) responses in autologous CD8+T cells from late-stage HIV-1-infected patients on prolonged combination drug therapy. Interleukin-12 enhanced this effect through an interleukin-2- and IFN-γ-mediated pathway. This suggests that dendritic cells from HIV-1-infected persons can be engineered to evoke stronger anti-HIV-1 CD8+T-cell reactivity as a strategy to augment antiretroviral therapy.Keywords
This publication has 21 references indexed in Scilit:
- CD8+T-Cell Gamma Interferon Production Specific for Human Immunodeficiency Virus Type 1 (HIV-1) in HIV-1-Infected SubjectsClinical and Diagnostic Laboratory Immunology, 2000
- HIV-1–specific immune responses in subjects who temporarily contain virus replication after discontinuation of highly active antiretroviral therapyJournal of Clinical Investigation, 1999
- T lymphocyte responses in HIV-1 infection: implications for vaccine developmentCurrent Opinion in Immunology, 1999
- Delivery of Liposome-Encapsulated HIV Type 1 Proteins to Human Dendritic Cells for Stimulation of HIV Type 1-Specific Memory Cytotoxic T Lymphocyte ResponsesAIDS Research and Human Retroviruses, 1999
- Functional T Cell Reconstitution and Human Immunodeficiency Virus–1–Specific Cell‐Mediated Immunity during Highly Active Antiretroviral TherapyThe Journal of Infectious Diseases, 1999
- Dendritic CellsPublished by Elsevier ,1999
- Anti-HIV Type 1 Memory Cytotoxic T Lymphocyte Responses Associated with Changes in CD4+T Cell Numbers in Progression of HIV Type 1 InfectionAIDS Research and Human Retroviruses, 1998
- Proinflammatory and Immunoregulatory Functions of Interleukin-12International Reviews of Immunology, 1998
- IL-12 in conjunction with dendritic cells enhances antiviral CD8+ CTL responses in vitro.Journal of Clinical Investigation, 1996
- Kinetics of Gag-specific cytotoxic T lymphocyte responses during the clinical course of HIV-1 infection: a longitudinal analysis of rapid progressors and long-term asymptomatics.The Journal of Experimental Medicine, 1995