Glucose Oxidase as an Analytical Reagent
- 1 September 1995
- journal article
- Published by Taylor & Francis in Critical Reviews in Analytical Chemistry
- Vol. 25 (1) , 1-42
- https://doi.org/10.1080/10408349508050556
Abstract
Glucose oxidase (EC 1.1.3.4) is the most widely employed enzyme as analytical reagent. This is the result of (1) its utility in the determination of glucose, an analyte of wide analytical interest, and (2) its relatively low cost and good stability that make the glucose/glucose oxidase system a very convenient model for method development (particularly in the area of biosensors). This review discusses in detail enzyme structure, biocatalysis, enzymes as analytical reagents, properties of glucose oxidase (including a historical account), and the use of glucose oxidase as an analytical reagent in homogeneous systems as well as an immobilized reagent.Keywords
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