Ultrastructural, histochemical, and freeze‐fracture evaluation of multilamellated structures in human pulmonary alveolar proteinosis

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Abstract
Ultrastructural, histochemical, and freeze‐fracture studies of material recovered by bron‐choalveolar lavage from patients with pulmonary alveolar proteinosis revealed four types (A, B, C, and D) of multilamellated structures (MS). Type A, the major component, consisted of concentric, trilaminar structures which were composed of two electron‐dense layers and a central lucent layer (5.7–7.5 nm in overall width) alternating with wider (25–30 nm) electron‐lucent intervening layers. Type B MS were formed by concentric lamellae with a 5–5.3‐nm periodicity. Type C MS were composed of wavy, electron‐dense lamellae with a 4–4.5‐nm periodicity. Type D MS were conglomerated masses of intricately arranged double or triple electron‐dense layers (7.5–13.5 nm wide) alternating with wider (30–40‐nm) electron‐lucent layers. The electron‐dense lamellae of type A, type C, and type D MS were stained with ruthenium red, the Thiéry method, and concanavalin A, indicating the presence of carbohydrate components. Freeze‐fracture studies revealed smooth inner and outer surfaces in type A MS, with the fracture planes passing through the central parts of the trilaminar structures; the intervening layers contained 10‐nm particles, which probably are proteins. Type B MS had smooth surfaces, and type C MS had slightly particulate surfaces; while type D MS showed tubular or polygonal structures, 350 nm wide, with rows of particles 7–8 nm in diameter. It is concluded that type A and type D MS contain proteins and carbohydrates, probably in the form of glycoproteins, as well as phos‐pholipids, and are related to tubular myelin. Type B and type C MS are considered to contain mainly phos‐pholipids; type C MS are also considered to contain carbohydrates and to be related to lamellar bodies of type II alveolar epithelial cells.