Molecular‐level pursuit of yeast mitosis by time‐ and space‐resolved Raman spectroscopy
Open Access
- 17 December 2002
- journal article
- research article
- Published by Wiley in Journal of Raman Spectroscopy
- Vol. 34 (1) , 1-3
- https://doi.org/10.1002/jrs.960
Abstract
The process of eukaryotic cell division has been studied at the molecular level by Raman spectroscopy. Time‐ and space‐resolved Raman spectra of a living fission yeast cell were successfully recorded with a 100 s time resolution and a 250 nm spatial resolution by using a confocal multi‐channel Raman microspectrometer. Raman spectra characteristic of the nucleus, mitochondrion and septum were identified and time‐dependent changes of major molecular components during the cell cycle were elucidated. Copyright © 2002 John Wiley & Sons, Ltd.Keywords
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