EVOCATION AND PERSISTENCE OF TRANSPLANTATION IMMUNITY IN RATS1

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Abstract
An investigation has been made of the sensitizing capacity in rats of orthotopic skin homografts of approximately constant size on the one hand, and of various dosages of dissociated cellular homografts administered by different routes on the other. As in mice differing by strong histocompatibility factors, primary orthotopic skin homografts are destroyed within 2 weeks; secondary grafts within 1 week; the immunity evoked by orthotopic skin homografts is long-lasting; that elicited by low dosages of dissociated cells is short-lived and dosage dependent. The i.p. route was found to be the most effective for immunization; sensitization evoked intradermally had a longer latency and duration; the i.v. route was effective only with relatively large numbers of cells; and the s.c. route was ineffective. Freund''s adjuvant did not facilitate the evocation of sensitivity by subcutaneously inoculated splenic cells, and epidermal cell suspensions were less effective on a dosage basis than spleen cells.