TPA (12-O-TETRADECANOYL-PHORBOL-13-ACETATE) ACTIVATION AND DIFFERENTIATION OF HUMAN PERIPHERAL LYMPHOCYTES-B

  • 1 January 1984
    • journal article
    • research article
    • Vol. 51  (1) , 27-34
Abstract
The effect of the tumor-promoting phorbol ester TPA (12-O-tetradecanoyl-phorbol-13-acetate) on normal human peripheral blood and tonsil B lymphocytes was investigated. A strong DNA-synthesis response with the maximum at day 4 was detected. This response was inhibited by increasing concentrations of serum in the medium. The membrane Ig expression was changed with a rapid decrease in IgD expression and a slower decrease in IgM and IgG expression. TPA-induced Ig secretion was detected in 12 of 22 tested donors and the response was independent of T cells and macrophages. The expression of 4 monoclonal antibody-detected B cell activation and differentiation markers, B1, B2, LB1 and BB1, was followed. The results indicate activation and differentiation of the B cells.