Abstract
Saccharin reacts quantitatively with Azure B in disodium hydrogen orthophosphate - citric acid buffer, forming a blue product that can be extracted into chloroform. Beer's law is valid over the concentration range 2–68 µg ml–1 of saccharin and the molar absorptivity is 2.4 × 103 l mol–1 cm–1. Reasonable amounts of ingredients that are likely to be present in soft drinks and pharmaceuticals do not interfere. Recoveries of saccharin from soft drinks and pharmaceuticals were satisfactory.

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