ENZYMATIC CHARACTERIZATION OF 17l-ARABINOSE NEGATIVE MUTANTS OFESCHERICHIA COLI
Open Access
- 1 June 1961
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 81 (6) , 996-1006
- https://doi.org/10.1128/jb.81.6.996-1006.1961
Abstract
Seventeen arabinose non-utilizing mutants of Escherichia coli strain B/r, previously ordered by genetic criteria and characterized by growth and accumulation experiments, have been defined enzymatically. Group A mutants are deficient in the enzyme L-arabinose isomerase and possess large amounts of L-ribulokinase, ranging from two to five times the activity shown by the wild type grown under similar conditions. Group B mutants are deficient in L-ribulokinase activity and have varying levels of L-arabinose isomerase activity, ranging from one-sixth to four times the wild type activity. Group C mutants are deficient in both isomerase and kinase activity. The grouping by enzymatic criteria corresponds exactly to the grouping by physiological and genetic criteria. Implications of the dual enzymatic effects in the various loci are discussed.Keywords
This publication has 24 references indexed in Scilit:
- ANTAGONISM BETWEEN SUBSTRATE AND REPRESSOR IN CONTROLLING THE FORMATION OF A BIOSYNTHETIC ENZYMEProceedings of the National Academy of Sciences, 1960
- The Genetic Control of the Enzymes of Histidine Biosynthesis in Salmonella typhimuriumJournal of General Microbiology, 1960
- HEREDITARY DEFECTS IN GALACTOSE METABOLISM IN ESCHERICHIA COLI MUTANTS, I. DETERMINATION OF ENZYME ACTIVITIESProceedings of the National Academy of Sciences, 1959
- Determination of the order of mutational sites governing l-arabinose utilization in Escherichia coli by transduction with phage P1btVirology, 1959
- GLUCOSE INHIBITION AND THE DIAUXIE PHENOMENONProceedings of the National Academy of Sciences, 1959
- COORDINATE REPRESSION OF THE SYNTHESIS OF FOUR HISTIDINE BIOSYNTHETIC ENZYMES BY HISTIDINEProceedings of the National Academy of Sciences, 1959
- Transduction and recombination study of linkage relationships among the genes controlling tryptophan synthesis in Escherichia coliVirology, 1959
- The genetic control and cytoplasmic expression of “Inducibility” in the synthesis of β-galactosidase by E. coliJournal of Molecular Biology, 1959
- Physiological basis for rhamnose utilization by a mutant of Pasteurella pestisArchives of Biochemistry and Biophysics, 1957
- Further evidence indicating the specificity of the orcinol spray reagent for ketoheptoses on paper chromatogramsArchives of Biochemistry and Biophysics, 1951