SIMPLE METHOD FOR ESTIMATION OF AMYGDALIN IN URINE

  • 1 January 1978
    • journal article
    • research article
    • Vol. 20  (2) , 367-378
Abstract
A procedure for the estimation of D- and D.L-amygdalin [an antineoplastic drug] in urine was described. Amygdalin was hydrolyzed by .beta.-glucosidase and base to benzaldehyde, glucose and cyanide. Benzaldehyde was extracted with methylene chloride and the UV absorbance determined at 243 nm. The response of human urine spiked with amygdalin was linear between 10 and 75 .mu.g/ml. Mice administered 100 mg/kg amygdalin i.v. or orally excreted about 70 and 20% of the administered dose, respectively, over 96 h. In each instance, more than 96% of excreted drug equivalents were obtained within the first 24 h.

This publication has 1 reference indexed in Scilit: