The role of the N‐terminal domain of chloroplast targeting peptides in organellar protein import and miss‐sorting
Open Access
- 19 June 2006
- journal article
- research article
- Published by Wiley in FEBS Letters
- Vol. 580 (16) , 3966-3972
- https://doi.org/10.1016/j.febslet.2006.06.018
Abstract
We have analysed 385 mitochondrial and 567 chloroplastic signal sequences of proteins found in the organellar proteomes ofArabidopsis thaliana. Despite overall similarities, the first 16 residues of transit peptides differ remarkably. To test the hypothesis that the N‐terminally truncated transit peptides would redirect chloroplastic precursor proteins to mitochondria, we studied import of the N‐terminal deletion mutants of ELIP, PetC and Lhcb2.1. The results show that the deletion mutants were neither imported into chloroplasts nor miss‐targeted to mitochondriain vitroandin vivo, showing that the entire transit peptide is necessary for correct targeting as well as miss‐sorting.Keywords
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