Induction of vanilloid receptor channel activity by protein kinase C
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- 1 December 2000
- journal article
- Published by Springer Nature in Nature
- Vol. 408 (6815) , 985-990
- https://doi.org/10.1038/35050121
Abstract
Capsaicin or vanilloid receptors (VRs) participate in the sensation of thermal and inflammatory pain. The cloned (VR1) and native VRs are non-selective cation channels directly activated by harmful heat, extracellular protons and vanilloid compounds. However, considerable attention has been focused on identifying other signalling pathways in VR activation; it is known that VR1 is also expressed in non-sensory tissue and may mediate inflammatory rather than acute thermal pain. Here we show that activation of protein kinase C (PKC) induces VR1 channel activity at room temperature in the absence of any other agonist. We also observed this effect in native VRs from sensory neurons, and phorbol esters induced a vanilloid-sensitive Ca2+ rise in these cells. Moreover, the pro-inflammatory peptide, bradykinin, and the putative endogenous ligand, anandamide, respectively induced and enhanced VR activity, in a PKC-dependent manner. These results suggest that PKC may link a range of stimuli to the activation of VRs.Keywords
This publication has 26 references indexed in Scilit:
- Vanilloid receptor-1 is essential for inflammatory thermal hyperalgesiaNature, 2000
- Impaired Nociception and Pain Sensation in Mice Lacking the Capsaicin ReceptorScience, 2000
- Distribution of mRNA for vanilloid receptor subtype 1 (VR1), and VR1-like immunoreactivity, in the central nervous system of the rat and humanProceedings of the National Academy of Sciences, 2000
- A Novel Nociceptor Signaling Pathway Revealed in Protein Kinase C ε Mutant MiceNeuron, 1999
- The Cloned Capsaicin Receptor Integrates Multiple Pain-Producing StimuliNeuron, 1998
- The capsaicin receptor: a heat-activated ion channel in the pain pathwayNature, 1997
- A novel heat-activated current in nociceptive neurons and its sensitization by bradykininProceedings of the National Academy of Sciences, 1996
- Capsaicin activates a nonselective cation channel in cultured neonatal rat dorsal root ganglion neuronsJournal of Neuroscience, 1996
- Ruthenium red blocks the capsaicin-induced increase in intracellular calcium and activation of membrane currents in sensory neurones as well as the activation of peripheral nociceptors in vitroNeuroscience Letters, 1990
- Capsaicin-induced ion fluxes in dorsal root ganglion cells in cultureJournal of Neuroscience, 1988