Isotope effect in peroxidation of deuterium-labelled ethanol by liver catalase
- 1 November 1980
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 191 (2) , 613-618
- https://doi.org/10.1042/bj1910613
Abstract
The kinetic V/K isotope effect upon peroxidation of (1R)-[1-2H1]ethanol by [rat] liver catalase was studied by using a radiochemical assay with both 3H and 14C as tracers. The value of 1.9 found is in agreement with the value of 2.52 for peroxidation of (1R)-[1-3H1]ethanol according to the Swain equation. There was no isotope effect on Vmax, the maximal rate of peroxidation of (1R)-[1-2H1]ethanol, at rates of H2O2 generation/catalase heme up to 500 min-1. Even at rates 20-fold higher, the isotope effect on Vmax may not differ from 1.This publication has 13 references indexed in Scilit:
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