Isolation and characterization of a high molecular weight antibiotic produced by a marine bacterium
- 1 December 1977
- journal article
- research article
- Published by Springer Nature in Microbial Ecology
- Vol. 3 (4) , 289-303
- https://doi.org/10.1007/bf02010737
Abstract
Many marine bacteria demonstrate antibiotic activity against organisms of terrestrial origin. Low molecular weight antibiotics have been extracted and, in some cases, purified, but few attempts have been made to isolate high molecular weight antibiotics produced by marine bacteria. In the study reported here, a high molecular weight antibiotic was extracted from whole cells ofAlteromonas strain P18 (NCMB 1890) grown on 2216E medium. Purification included ammonium sulfate precipitation, ultracentrifugation, chromatography on DEAE cellulose, and gel filtration on Ultrogel. A rapid method for measuring specific activity of the antibiotic was developed.Keywords
This publication has 27 references indexed in Scilit:
- Glycoprotein staining following electrophoresis on acrylamide gelsPublished by Elsevier ,2004
- Modification of Bacterial Respiration by a Macromolecular Polyanionic Antibiotic Produced by a Marine AlteromonasAntimicrobial Agents and Chemotherapy, 1976
- Taxonomic Position and Seasonal Variations in Marine Neritic Environment of Some Gram-negative Antibiotic-producing BacteriaJournal of General Microbiology, 1975
- Chapter VII Methods for Studying BacteriocinsPublished by Elsevier ,1972
- Study of bacteria associated with marine algae in cultureMarine Biology, 1969
- Lysis of Escherichia coli by Marine Micro-organismsNature, 1967
- DISC ELECTROPHORESIS – II METHOD AND APPLICATION TO HUMAN SERUM PROTEINS*Annals of the New York Academy of Sciences, 1964
- DISC ELECTROPHORESIS‐I BACKGROUND AND THEORY*Annals of the New York Academy of Sciences, 1964
- Heterotrophic Bacteria in Marine Environments of Eastern AustraliaMarine and Freshwater Research, 1953
- Viability of Escherichia coli in Sea WaterAmerican Journal of Public Health and the Nations Health, 1950