Protein gene product 9.5 and ubiquitin immunoreactivities in rat epididymis epithelium
- 1 August 1993
- journal article
- research article
- Published by Springer Nature in Histochemistry and Cell Biology
- Vol. 100 (2) , 131-138
- https://doi.org/10.1007/bf00572899
Abstract
A quantitative immunohistochemical study was performed of the distribution of protein gene product 9.5 (PGP, a soluble protein localized in neurons and neuroendocrine cells as well as in some non-nervous cells) and ubiquitin along the rat epididymis. In the ductuli efferents, PGP immunoreaction was observed in the whole cytoplasm of some columnar cells; a smaller number of columnar cells showed ubiquitin immunoreactivity with limited apical and basal cytoplasmic localization. In the proximal caput epididymidis, the whole cytoplasm of all columnar cells showed PGP immunoreactivity, ubiquitin immunostaining was negative in this region. In the middle and distal caput epididymidis and the distal cauda, the apical cytoplasm of some columnar cells and the whole cytoplasm of some basal cells showed immunoreactivity to PGP. In these regions, immunoreactivity to ubiquitin was positive in the supranuclear cytoplasm of some columnar cells but not in the basal cells. No immunoreactivity to PGP or ubiquitin was detected in the corpus epididymis and the proximal cauda. Double immunostaining revealed that all the epididymal ubiquitin immunoreactive cells were also PGP immunoreactive, whereas most PGP immunoreactive cells did not immunoreact to ubiquitin. In ubiquitin-PGP immunoreactive cells, the site of the PGP immunoreaction differed from that of the ubiquitin immunoreaction. PGP-ubiquitin immunoreactive cells also seemed to be immunoreactive to anti-AE1/AE3 keratin antibodies. The spermatozoal heads were immunoreactive to PGP antibodies in the epididymal regions from proximal caput to distal cauda but not in the ductuli efferents. The findings suggest that non-ubiquitinated PGP immunoreactive proteins are secreted in the epididymis, mainly in the proximal caput, and attach to spermatozoa.Keywords
This publication has 47 references indexed in Scilit:
- ‘Neuron‐specific’ protein gene product 9.5 (PGP 9.5) is also expressed in glioma cell lines and its expression depends on cellular growth stateFEBS Letters, 1991
- Organization of tubules in the human caput epididymidis and the ultrastructure of their epitheliaJournal of Anatomy, 1991
- Immunogold localisation of ubiquitin‐protein conjugates in primary (azurophilic) granules of polymorphonuclear neutrophilsFEBS Letters, 1991
- Double and triple immunocytochemical labelling at the light microscope level in histopathologyJournal of Molecular Histology, 1990
- Two-Dimensional Electrophoresis of Proteins in Principal Cells, Spermatozoa, and Fluid Associated with the Rat EpididymisBiology of Reproduction, 1987
- Color modification of diaminobenzidine (DAB) precipitation by metallic ions and its application for double immunohistochemistry.Journal of Histochemistry & Cytochemistry, 1982
- Proliferative activity in the rat epididymis during postnatal developmentThe Anatomical Record, 1982
- Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.Proceedings of the National Academy of Sciences, 1979
- The differential absorptive activity of epithelial cells of the rat epididymus before and after castrationThe Anatomical Record, 1979
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970