Cysteine Proteinase-Inhibitory Activity ofrasGene Product is not Affected by Mutations at GTPase-Activating Protein-Binding Sites
- 1 January 1989
- journal article
- research article
- Published by Walter de Gruyter GmbH in Biological Chemistry Hoppe-Seyler
- Vol. 370 (2) , 1215-1220
- https://doi.org/10.1515/bchm3.1989.370.2.1215
Abstract
We have investigated the proteinase-inhibitory activity against cathepsin L of some c-Ha-ras gene products (p21s) with point mutations at position 12, 33 or 35, which affect the interaction with GTPase-activating protein (GAP). All of the full-length p21s examined showed similar inhibitory activities irrespective of the point mutation and the transforming activity. These results suggest that mutations at GAP-interacting sites have no effect on the proteinase-inhibitory activity of p21s and that the proteinase-inhibitory activity alone is not sufficient for the transformation caused by p21s.This publication has 20 references indexed in Scilit:
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