Competition between splicing and polyadenylation reactions determines which adenovirus region E3 mRNAs are synthesized.
Open Access
- 1 August 1988
- journal article
- research article
- Published by Taylor & Francis in Molecular and Cellular Biology
- Vol. 8 (8) , 3291-3297
- https://doi.org/10.1128/mcb.8.8.3291
Abstract
Complex transcription units encode multiple mRNAs which arise by alternative processing of a common pre-mRNA precursor. It is not known how the pre-mRNA processing pathways are determined or controlled. We are investigating this problem by using the E3 complex transcription unit of adenovirus as a model. Our approach is to construct virus mutants with lesions in E3 and then determine how the mutation affects the accumulation of E3 mRNAs in vivo. We report results which indicate that competition between splicing reactions and polyadenylation reactions occurs in vivo and that this plays an important role in alternative pre-mRNA processing.This publication has 35 references indexed in Scilit:
- A 64 kd nuclear protein binds to RNA segments that include the AAUAAA polyadenylation motifCell, 1988
- Electrophoretic separation of polyadenylation-specific complexes.Genes & Development, 1987
- ALTERNATIVE SPLICING: A UBIQUITOUS MECHANISM FOR THE GENERATION OF MULTIPLE PROTEIN ISOFORMS FROM SINGLE GENESAnnual Review of Biochemistry, 1987
- A protein that specifically recognizes the 3′ splice site of mammalian pre-mRNA introns is associated with a small nuclear ribonucleoproteinCell, 1986
- Deletion mutants that alter differential RNA processing in the E3 complex transcription unit of adenovirusJournal of Molecular Biology, 1986
- A small nuclear ribonucleoprotein associates with the AAUAAA polyadenylation signal in vitroCell, 1986
- The 3′ Splice Site of Pre-Messenger RNA Is Recognized by a Small Nuclear RibonucleoproteinScience, 1985
- Specific and stable intron-factor interactions are established early during in vitro pre-mRNA splicingCell, 1985
- α-Thalassaemia caused by a polyadenylation signal mutationNature, 1983
- Inhibition of RNA cleavage but not polyadenylation by a point mutation in mRNA 3′ consensus sequence AAUAAANature, 1983