Ethanol production from glucose and dilute‐acid hydrolyzates by encapsulated S. cerevisiae
- 16 March 2005
- journal article
- research article
- Published by Wiley in Biotechnology & Bioengineering
- Vol. 90 (3) , 345-353
- https://doi.org/10.1002/bit.20432
Abstract
The performance of encapsulated Saccharomyces cerevisiae CBS 8066 in anaerobic cultivation of glucose, in the presence and absence of furfural as well as in dilute‐acid hydrolyzates, was investigated. The cultivation of encapsulated cells in 10 sequential batches in synthetic media resulted in linear increase of biomass up to 106 g/L of capsule volume, while the ethanol productivity remained constant at 5.15 (±0.17) g/L·h (for batches 6–10). The cells had average ethanol and glycerol yields of 0.464 and 0.056 g/g in these 10 batches. Addition of 5 g/L furfural decreased the ethanol productivity to a value of 1.31 (±0.10) g/L·h with the encapsulated cells, but it was stable in this range for five consecutive batches. On the other hand, the furfural decreased the ethanol yield to 0.41–0.42 g/g and increased the yield of acetic acid drastically up to 0.068 g/g. No significant lag phase was observed in any of these experiments. The encapsulated cells were also used to cultivate two different types of dilute‐acid hydrolyzates. While the free cells were not able to ferment the hydrolyzates within at least 24 h, the encapsulated yeast successfully converted glucose and mannose in both of the hydrolyzates in less than 10 h with no significant lag phase. However, since the hydrolyzates were too toxic, the encapsulated cells lost their activity gradually in sequential batches.Keywords
This publication has 31 references indexed in Scilit:
- Carrageenan–oligochitosan microcapsules: optimization of the formation processColloids and Surfaces B: Biointerfaces, 2001
- Alginate−Oligochitosan Microcapsules: A Mechanistic Study Relating Membrane and Capsule Properties to Reaction ConditionsChemistry of Materials, 1999
- Microencapsulation of recombinantSaccharomyces cerevisiae cells with invertase activity in liquid-core alginate capsulesBiotechnology & Bioengineering, 1996
- Microencapsulation of yeast cells and their use as a biocatalyst in organic solventsBiotechnology & Bioengineering, 1996
- Immobilization of Cells for Application in the Food IndustryCritical Reviews in Biotechnology, 1994
- Microencapsulation of yeast cells in the calcium alginate membraneBiotechnology Techniques, 1993
- The effects of furfural on ethanol production by saccharomyces cereyisiae in batch cultureBiomass and Bioenergy, 1992
- Growing Saccharomyces cerevisiae in calcium‐alginate beads induces cell alterations which accelerate glucose conversion to ethanolBiotechnology & Bioengineering, 1990
- Ethanol fermentation of crude acid hydrolyzate of cellulose using high‐level yeast inoculaBiotechnology & Bioengineering, 1985
- Inhibition of glycolysis by furfural in Saccharomyces cerevisiaeApplied Microbiology and Biotechnology, 1981