Complete amino acid sequence of human placental 17β‐hydroxysteroid dehydrogenase deduced from cDNA
- 19 October 1988
- journal article
- Published by Wiley in FEBS Letters
- Vol. 239 (1) , 73-77
- https://doi.org/10.1016/0014-5793(88)80548-9
Abstract
CDNA clones for 17β‐hydroxysteroid dehydrogenase (17‐HSD; EC 1.1.1.62) were isolated from a placental λgt11 expression library using polyclonal antibodies against placental 17‐HSD. The largest cDNA contained 1325 nucleotides, consisting of a short 5′‐noncoding segment, a coding segment of 987 nucleotides terminated by a TAA codon, and a 329 nucleotide long 3′‐noncoding segment. The open reading frame encoded a polypeptide of 327 amino acid residues with a predicted M r of 34853. The amino acid sequence of 23 N‐terminal amino acids determined from purified 17‐HSD agreed with the sequence deduced from cDNA. The deduced amino acid sequence also contained two peptides previously characterized from the proposed catalytic area of placental 17‐HSD.Keywords
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